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locigenesis

locigenesis is a tool that generates an immune repertoire and runs it through a sequence reader simulation tool, to generate sequencing errors.

Installation

This project uses Nix to ensure reproducible builds.

  1. Install Nix (compatible with MacOS, Linux and WSL):
curl -L https://nixos.org/nix/install | sh
  1. Clone the repository:
git clone https://git.coolneng.duckdns.org/coolneng/locigenesis
  1. Change the working directory to the project:
cd locigenesis
  1. Enter the nix-shell:
nix-shell

After running these commands, you will find yourself in a shell that contains all the needed dependencies.

Usage

An execution script that accepts 2 parameters is provided, the following command invokes it:

./generation.sh <number of sequences> <number of reads>
  • <number of sequences>: an integer that specifies the number of different sequences to generate
  • <number of reads>: an integer that specifies the number of reads to perform on each sequence

The script will generate 2 files under the data directory:

HVR.fastq Contains the original CDR3 sequence
CuReSim-HVR.fastq Contains CDR3 after the read simulation, with sequencing errors
Description
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